Page: 4 Pages
PDF Source: www.biochemj.org
Tags: galactosamine, Glucosamine
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The Determination of Glucosamine and Galactosamine. An analytical procedure for the quantitative determination of glucosamine and galactosamine was described by Elson & Morgan (1933).An analytical procedure for the quantitative determination of glucosamine and galactosamine was described by Elson & Morgan (1933). The method was based on the observation by Pauly & Ludwig (1922) that in alkaline solution at 100° the amino sugars react with acetylacetone to form a chromogenic material which gives a chromophore or chromophores on treatment in acid solution with ethanolic p-dimethylaminobenzaldehyde. The development and use of photoelectric spectrophoto-meters during the intervening years has increased the sensitivity and accuracy of technique very considerably, but although many modifications of procedure designed to improve the method have been described there has, in fact, been no funda- mental change in the procedure originally employed.